|
2011.3.29 福建農林菌類實驗室（伯樂 餘小菁 陳洪亮） |
09:00-10:00 |
MIQE Guidelines |
Minimum Information for Publication of qPCR Experiments |
10:00-10:30 |
Assay Design Considerations, Optimization and Validation |
Primers and Probes Designs
Assay Validation
Gradient
|
10:30-10:45 |
Break |
10:45-11:20 |
Sample Preparation & Quality Control |
Biology & Chemistry
DNA & RNA Isolation
|
11:20-12:00 |
q PCR Chemistry |
Taqman
SYBR Green
Molecular Beacons
Dual-Oligo FRET Probes
|
12:00-13:30 |
Lauch |
|
13:30-14:00
|
Practical course |
Use Ssofast Eva Green supermix to validate and optimize qPCR |
14:00:14:30 |
Superior qPCR Reagent and plastic consumable from Bio-Rad
|
By hongliangchen |
14:30-15:30 |
Practical course data analysis
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Standard curve and melt curve |
15:30-16:00 |
Break |
16:00-16:30 |
Relative Quantifications: Data Management & Analysis Settings
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Housekeeping genes
Controls
|
16:30-17:00 |
Discussion |
